ARG56954
anti-Hsp 105 antibody [J1G12]
anti-Hsp 105 antibody [J1G12] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [J1G12] recognizes Hsp 105 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | J1G12 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | Hsp 105 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-858 of Human Hsp 105. |
| 偶联标记 | Un-conjugated |
| 別名 | HSP105B; HSP105A; Heat shock protein 105 kDa; HSP105; Heat shock 110 kDa protein; NY-CO-25; Antigen NY-CO-25 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSPH1 |
| 全名 | heat shock 105kDa/110kDa protein 1 |
| 生物功能 | Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity). [UniProt] |
| 预测分子量 | 97 kDa |
| 翻译后修饰 | Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG56954 anti-Hsp 105 antibody [J1G12] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56954 anti-Hsp 105 antibody [J1G12] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56954 anti-Hsp 105 antibody [J1G12] WB image
Western blot: 40 µg of 1) HeLa, 2) HepG2, 3) NIH-3T3, 4) MCF7, 5) U-87 MG, and 6) A549 cell lysates stained with ARG56954 anti-Hsp 105 antibody [J1G12] at 1:1000.
