ARG55373
anti-Hsc 70 antibody
anti-Hsc 70 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Hsc 70 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Bov, Chk, Hm |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Hsc 70 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 82-110 (N-terminus) of Human Hsc 70. |
| 偶联标记 | Un-conjugated |
| 別名 | NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | A431 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSPA8 |
| 全名 | heat shock 70kDa protein 8 |
| 背景介绍 | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| 生物功能 | Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP. [UniProt] |
| 细胞定位 | Cytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 71 kDa |
| 翻译后修饰 | Acetylated. ISGylated. Trimethylation at Lys-561 reduces fibrillar SNCA binding. |
检测图片 (4) Click the Picture to Zoom In
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ARG55373 anti-Hsc 70 antibody ICC/IF image
Immunofluorescence: A2058 cells stained with ARG55373 anti-Hsc 70 antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI (blue) for nuclear staining.
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ARG55373 anti-Hsc 70 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG55373 anti-Hsc 70 antibody.
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ARG55373 anti-Hsc 70 antibody WB image
Western blot: 35 µg of A431 cell lysate stained with ARG55373 anti-Hsc 70 antibody at 1:1000 dilution.
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ARG55373 anti-Hsc 70 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG55373 anti-Hsc 70 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
