ARG41926
anti-Histone H1.3 + H1.4 phospho (Thr17) antibody
anti-Histone H1.3 + H1.4 phospho (Thr17) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Histone H1.3 + H1.4 phospho (Thr17) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Histone H1.3 + H1.4 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Thr17 of Human Histone H1.3 / H1.4. |
| 偶联标记 | Un-conjugated |
| 別名 | Histone H1s-4; Histone H1.4; H1F4; H1s-4; H1.4; Histone H1b; dJ221C16.5; H1E |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat | ||||||||
| 实际分子量 | ~ 31 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HIST1H1E |
| 全名 | histone cluster 1, H1e |
| 背景介绍 | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| 生物功能 | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Chromosome. Note=Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus. [UniProt] |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. ADP-ribosylated on Ser-150 in response to DNA damage. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41926 anti-Histone H1.3 + H1.4 phospho (Thr17) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse colon tissue stained with ARG41926 anti-Histone H1.3 + H1.4 phospho (Thr17) antibody.
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ARG41926 anti-Histone H1.3 + H1.4 phospho (Thr17) antibody WB image
Western blot: Jurkat cell lysate stained with ARG41926 anti-Histone H1.3 + H1.4 phospho (Thr17) antibody.
