ARG63821
anti-Histamine Receptor H2 antibody
anti-Histamine Receptor H2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Histamine Receptor H2 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Dog |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Histamine Receptor H2 |
| 抗原物种 | Human |
| 抗原 | C-QEEKPLKLQVWSGTE |
| 偶联标记 | Un-conjugated |
| 別名 | HH2R; Histamine H2 receptor; Gastric receptor I; H2R |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 40 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG63821 anti-Histamine Receptor H2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63821 anti-Histamine Receptor H2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63821 anti-Histamine Receptor H2 antibody WB image
Western blot: 35 µg of Human tonsil lysate (in RIPA buffer) stained with ARG63821 anti-Histamine Receptor H2 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63821 anti-Histamine Receptor H2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63821 anti-Histamine Receptor H2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
