ARG42871
anti-HYOU1 / ORP150 antibody
anti-HYOU1 / ORP150 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HYOU1 / ORP150 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HYOU1 / ORP150 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human HYOU1 / ORP150. |
| 偶联标记 | Un-conjugated |
| 別名 | GRP-170; Hypoxia up-regulated protein 1; Grp170; ORP150; 150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; HSP12A; ORP-150 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | HeLa | ||||||||||
| 实际分子量 | ~ 150 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HYOU1 |
| 全名 | hypoxia up-regulated 1 |
| 背景介绍 | The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
| 生物功能 | Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. [UniProt] |
| 细胞定位 | Endoplasmic reticulum lumen. [UniProt] |
| 预测分子量 | 111 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG42871 anti-HYOU1 / ORP150 antibody WB image
Western blot: HeLa cell lysate stained with ARG42871 anti-HYOU1 / ORP150 antibody at 1:1000 dilution.
