ARG58859
anti-HUS1 antibody
anti-HUS1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HUS1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HUS1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-280 of Human HUS1 (NP_004498.1). |
| 偶联标记 | Un-conjugated |
| 別名 | hHUS1; Checkpoint protein HUS1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | 293T | ||||||||
| 实际分子量 | 35 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HUS1 |
| 全名 | HUS1 checkpoint clamp component |
| 背景介绍 | The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011] |
| 生物功能 | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. [UniProt] |
| 细胞定位 | Nucleus, Cytoplasm. [UniProt] |
| 预测分子量 | 32 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58859 anti-HUS1 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG58859 anti-HUS1 antibody.
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ARG58859 anti-HUS1 antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG58859 anti-HUS1 antibody at 1:1000 dilution.
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ARG58859 anti-HUS1 antibody IP image
Immunoprecipitation: 200 µg extracts of 293T cells were immunoprecipitated and stained with ARG58859 anti-HUS1 antibody at 1:1000 dilution.
