ARG45305
anti-HUCE1 antibody
anti-HUCE1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Polyclonal antibody recognizes HUCE1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HUCE1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human HUCE1. |
| 偶联标记 | Un-conjugated |
| 別名 | RRP8; Ribosomal RNA Processing 8; KIAA0409; NML; Nucleomethylin; Ribosomal RNA-Processing Protein 8; Cerebral Protein 1; Ribosomal RNA Processing 8, Methyltransferase, Homolog (Yeast); Ribosomal RNA Processing 8, Methyltransferase, Homolog; RRP8 Methyltransferase Homolog (S. Cerevisiae); RRP8 Methyltransferase Homolog; EC 2.1.1.- |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 65 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RRP8 |
| 全名 | Ribosomal RNA Processing 8 |
| 背景介绍 | Enables methylated histone binding activity. Involved in several processes, including cellular response to glucose starvation; intrinsic apoptotic signaling pathway by p53 class mediator; and regulation of gene expression. Acts upstream of with a negative effect on regulation of G1 to G0 transition. Located in several cellular components, including cytosol; nuclear lumen; and rDNA heterochromatin. Part of eNoSc complex. [provided by Alliance of Genome Resources, Nov 2024] |
| 生物功能 | Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Phosphoprotein. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45305 anti-HUCE1 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45305 anti-HUCE1 antibody at 5 ug/ml dilution.
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ARG45305 anti-HUCE1 antibody WB image
Western blot: MCF-7 stained with ARG45305 anti-HUCE1 antibody at 0.5 μg/ml dilution.
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ARG45305 anti-HUCE1 antibody FACS image
Flow Cytometry: Hela stained with ARG45305 anti-HUCE1 antibody at 1 µg/10^6 cells dilution.
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ARG45305 anti-HUCE1 antibody WB image
Western blot: Rat brain stained with ARG45305 anti-HUCE1 antibody at 0.5 μg/ml dilution.
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ARG45305 anti-HUCE1 antibody WB image
Western blot: Mouse brain stained with ARG45305 anti-HUCE1 antibody at 0.5 μg/ml dilution.
