ARG63296
anti-HSPC150 / UBE2T antibody
anti-HSPC150 / UBE2T antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes HSPC150 / UBE2T |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HSPC150 / UBE2T |
| 抗原物种 | Human |
| 抗原 | C-QLVGIEKKFHPDV |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.19; FANCT; PIG50; Cell proliferation-inducing gene 50 protein; Ubiquitin carrier protein T; HSPC150; Ubiquitin-conjugating enzyme E2 T; Ubiquitin-protein ligase T |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM, Mar 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 23 kDa |
| 翻译后修饰 | Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity (PubMed:16916645). In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity (PubMed:19111657). |
检测图片 (1) Click the Picture to Zoom In
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ARG63296 anti-HSPC150 / UBE2T antibody WB image
Western Blot: K562 lysate (35 µg protein in RIPA buffer) stained with ARG63296 anti-HSPC150 / UBE2T antibody at 0.1 µg/ml dilution.
