ARG58827
anti-HSF4 antibody
anti-HSF4 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HSF4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Bov |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HSF4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 149-185 of Human HSF4 (VQESTEARLRELRQQNEILWREVVTLRQSHGQQHRVI). |
| 偶联标记 | Un-conjugated |
| 別名 | CTRCT5; Heat shock transcription factor 4; HSF 4; Heat shock factor protein 4; HSTF 4; CTM; hHSF4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSF4 |
| 全名 | heat shock transcription factor 4 |
| 背景介绍 | Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293. Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG58827 anti-HSF4 antibody WB image
Western blot: 40 µg of HeLa and MCF-7 cell lysates stained with ARG58827 anti-HSF4 antibody at 0.5 µg/ml dilution.
