ARG58810
anti-HSF2 antibody
anti-HSF2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HSF2 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Hm |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HSF2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 82-102 of Human HSF2 (KQERDGPVEFQHPYFKQGQDD). |
| 偶联标记 | Un-conjugated |
| 別名 | HSF 2; Heat shock factor protein 2; Heat shock transcription factor 2; HSTF 2 |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSF2 |
| 全名 | heat shock transcription factor 2 |
| 背景介绍 | The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| 生物功能 | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation. [UniProt] |
| 预测分子量 | 60 kDa |
检测图片 (5) Click the Picture to Zoom In
-
ARG58810 anti-HSF2 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG58810 anti-HSF2 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
-
ARG58810 anti-HSF2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG58810 anti-HSF2 antibody.
-
ARG58810 anti-HSF2 antibody WB image
Western blot: 50 µg of Rat kidney, 50 µg of Rat Spleen, 40 µg of 293T, 40 µg of MCF-7, 40 µg of Jurkat, 40 µg of A549 and 40 µg of CEM lysates stained with ARG58810 anti-HSF2 antibody at 0.5 µg/ml dilution.
-
ARG58810 anti-HSF2 antibody FACS image
Flow Cytometry: 293T cells were blocked with 10% normal goat serum and then stained with ARG58810 anti-HSF2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG58810 anti-HSF2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG58810 anti-HSF2 antibody.
