anti-HRP antibody [SQab1887]

anti-HRP antibody [SQab1887] for Flow cytometry,ICC/IF,Western blot and Other


产品描述 Recombinant Rabbit Monoclonal antibody [SQab1887] recognizes HRP
反应物种 Other
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1887
同位型 IgG
靶点名称 HRP
抗原物种 Others
抗原 Full length native protein (purified) corresponding to HRP.
偶联标记 Un-conjugated


应用 推荐稀释比
WB1:5000 - 1:10000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.


预测分子量 39 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG66339 anti-HRP antibody [SQab1887] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66339 anti-HRP antibody [SQab1887] (green) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66339 anti-HRP antibody [SQab1887] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66339 anti-HRP antibody [SQab1887] (red) at 1:50 dilution in 1x PBS/1% BSA for 60 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66339 anti-HRP antibody [SQab1887] WB image

    Western blot: 20 ng of HRP protein stained with ARG66339 anti-HRP antibody [SQab1887] at 1:10000 dilution.