ARG10496
anti-HPV16 E7 antibody [716-D1]
anti-HPV16 E7 antibody [716-D1] for ELISA,Western blot and HPV
Microbiology and Infectious Disease antibody
概述
| 产品描述 | Mouse Monoclonal antibody [716-D1] recognizes HPV16 E7 |
|---|---|
| 反应物种 | HPV |
| 应用 | ELISA, WB |
| 特异性 | The clone 716-D1 can detect both monomer and dimmer forms of HPV type 16 E7 protein and it is also equally suitable for capture of both HPV type 16 and 18 according WB result. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 716-D1 |
| 同位型 | IgG2a |
| 靶点名称 | HPV16 E7 |
| 抗原物种 | Virus |
| 抗原 | Balb/c mice immunized with HPV oncoprotein E7, type 16. |
| 抗原表位 | a.a. 36-43 |
| 偶联标记 | Un-conjugated |
应用说明
| 应用说明 | ELISA: Recommended pair for sandwich immunoassay: ARG10496 (capture) and ARG11058 (detection) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G affinity purified. |
| 缓冲液 | PBS (pH 7.2) and 0.1% Sodium azide |
| 抗菌剂 | 0.1 % Sodium azide |
| 浓度 | 1.0-2.0 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | E7 |
| 全名 | transforming protein |
| 生物功能 | E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription (By similarity). [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30191 HPV18 E7 ELISA Antibody Duo Related products: HPV antibodies; HPV ELISA Kits; HPV Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Microbiology and Infectious Disease antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG10496 anti-HPV16 E7 antibody [716-D1] WB image
Western blot:15 µg of recominbinant protein stained with ARG10496 anti-HPV16 E7 antibody [716-D1].
