ARG63877
anti-HP1 alpha antibody
anti-HP1 alpha antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Controls and Markers antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes HP1 alpha |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog |
| 应用 | FACS, IHC-P, WB |
| 特异性 | Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HP1 alpha |
| 抗原物种 | Human |
| 抗原 | C-NKRKSNFSNSADDIK |
| 偶联标记 | Un-conjugated |
| 別名 | Heterochromatin protein 1 homolog alpha; Chromobox protein homolog 5; HP1; HP1 alpha; Antigen p25; HEL25; HP1A |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| 研究领域 | Controls and Markers antibody; Gene Regulation antibody |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. Ubiquitinated. |
检测图片 (3) Click the Picture to Zoom In
-
ARG63877 anti-HP1 alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63877 anti-HP1 alpha antibody at 2 µg/ml dilution followed by HRP-staining.
-
ARG63877 anti-HP1 alpha antibody WB image
Western blot: 35 µg of K562 (A), MCF7 (B) and NIH/3T3 (C) cell lysates (in RIPA buffer) stained with ARG63877 anti-HP1 alpha antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63877 anti-HP1 alpha antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63877 anti-HP1 alpha antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
