ARG53998
anti-HP1 alpha antibody
anti-HP1 alpha antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Controls and Markers antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes CBX5 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | HP1 alpha |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human HP1 alpha protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Heterochromatin protein 1 homolog alpha; Chromobox protein homolog 5; HP1; HP1 alpha; Antigen p25; HEL25; HP1A |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: High pressure mediate and boil tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 26 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CBX5 |
| 全名 | chromobox homolog 5 |
| 背景介绍 | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins |
| 生物功能 | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. [UniProt] |
| 细胞定位 | Nucleus. Chromosome |
| 研究领域 | Controls and Markers antibody; Gene Regulation antibody |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. Ubiquitinated. |
检测图片 (3) Click the Picture to Zoom In
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ARG53998 anti-HP1 alpha antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG53998 anti-HP1 alpha antibody at 1:300 dilution.
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ARG53998 anti-HP1 alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma stained with ARG53998 anti-HP1 alpha antibody at 1:400 dilution. Antigen Retrieval: High pressure mediate and boil tissue section in Citrate buffer (pH 6.0).
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ARG53998 anti-HP1 alpha antibody WB image
Western blot: 293T cell lysate stained with ARG53998 anti-HP1 alpha antibody at 1:1000 dilution.
