ARG51547
anti-HNF4A phospho (Ser304) antibody
anti-HNF4A phospho (Ser304) antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HNF4A phospho (Ser304) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HNF4A |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 304 (L-R-S(p)-Q-V)derived from Human HNF4α. |
| 偶联标记 | Un-conjugated |
| 別名 | Transcription factor HNF-4; HNF4a9; HNF4a8; Transcription factor 14; MODY; HNF4a7; HNF4alpha; TCF-14; TCF14; Nuclear receptor subfamily 2 group A member 1; MODY1; NR2A21; Hepatocyte nuclear factor 4-alpha; TCF; NR2A1; HNF4; FRTS4; HNF-4-alpha |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HNF4A |
| 全名 | hepatocyte nuclear factor 4, alpha |
| 背景介绍 | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes,including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants. |
| 生物功能 | Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold. |
检测图片 (1) Click the Picture to Zoom In
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ARG51547 anti-HNF4A phospho (Ser304) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with Anisomyclin stained with ARG51547 anti-HNF4A phospho (Ser304) antibody.
