ARG59687
anti-HMGN2 antibody
anti-HMGN2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HMGN2 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HMGN2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human HMGN2. (KKGKADAGKEGNNPAENGDAKTDQAQKAEGAGDAK) |
| 偶联标记 | Un-conjugated |
| 別名 | HMG17; High mobility group nucleosome-binding domain-containing protein 2; Non-histone chromosomal protein HMG-17 |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||
| 实际分子量 | 18 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P05204 Human Non-histone chromosomal protein HMG-17 |
|---|---|
| 基因名称 | HMGN2 |
| 全名 | high mobility group nucleosomal binding domain 2 |
| 背景介绍 | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi. [provided by RefSeq, Oct 2014] |
| 生物功能 | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. [UniProt] |
| 预测分子量 | 9 kDa |
| 翻译后修饰 | Phosphorylation favors cytoplasmic localization. [UniProt] |
检测图片 (10) Click the Picture to Zoom In
-
ARG59687 anti-HMGN2 antibody ICC/IF image
Immunofluorescence: MCF-7 cells were blocked with 10% goat serum and then stained with ARG59687 anti-HMGN2 antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
-
ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
-
ARG59687 anti-HMGN2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, A549 and U-87MG whole cell lysates stained with ARG59687 anti-HMGN2 antibody at 0.5 µg/ml, overnight at 4°C.
-
ARG59687 anti-HMGN2 antibody FACS image
Flow Cytometry: Raji cells were blocked with 10% normal goat serum and then stained with ARG59687 anti-HMGN2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oesophagus squama cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
-
ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
-
ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human appendicitis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
-
ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
-
ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
-
ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
