概述

产品描述	Rabbit Polyclonal antibody recognizes HMGCR
反应物种	Hu
预测物种	Ms, Rat
应用	IP, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	HMGCR
抗原物种	Human
抗原	Synthetic peptide derived from Human HMGCR.
偶联标记	Un-conjugated
別名	LDLCQ3; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; EC 1.1.1.34; HMG-CoA reductase

应用说明

应用建议	应用 推荐稀释比 IP 1:50 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	Jurkat
实际分子量	~ 98 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3156 Human HMGCR Swiss-port # P04035 Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase
基因名称	HMGCR
全名	3-hydroxy-3-methylglutaryl-CoA reductase
背景介绍	HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
生物功能	Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins. [UniProt]
细胞定位	Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
预测分子量	97 kDa
翻译后修饰	N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner. Undergoes sterol-mediated ubiquitination and ER-association degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1. This INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. [UniProt]

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