ARG63816
anti-HMGA1 antibody
anti-HMGA1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Gene Regulation antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes HMGA1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | IHC-P |
| 特异性 | This antibody is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HMGA1 |
| 抗原物种 | Human |
| 抗原 | C-LASKQEKDGTEK |
| 偶联标记 | Un-conjugated |
| 別名 | HMG-I; High mobility group protein HMG-I/HMG-Y; High mobility group protein A1; High mobility group protein R; Y; High mobility group AT-hook protein 1; HMG-R; HMGA1A; HMGIY |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17096 Human High mobility group protein HMG-I/HMG-Y |
|---|---|
| 背景介绍 | This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 12 kDa |
| 翻译后修饰 | Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. |
检测图片 (2) Click the Picture to Zoom In
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ARG63816 anti-HMGA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63816 anti-HMGA1 antibody at 4 µg/ml dilution followed by AP-staining.
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ARG63816 anti-HMGA1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Placenta. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63816 anti-HMGA1 antibody at 4 µg/ml dilution followed by AP-staining.
