ARG58911
anti-HMBS antibody
anti-HMBS antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes HMBS |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize all reported isoforms (NP_000181.2; NP_001019553.1; NP_001245137.1; NP_001245138.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HMBS |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from the internal region of Human HMBS. (NP_000181.2; NP_001019553.1; NP_001245137.1; NP_001245138.1) (C-HLEFRSIRGNLNTR) |
| 偶联标记 | Un-conjugated |
| 別名 | HMBS; Hydroxymethylbilane synthase; Porphobilinogen deaminase; PORC; PBGD; EC 2.5.1.61; Pre-uroporphyrinogen synthase; PBG-D; UPS |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 实际分子量 | ~ 40 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HMBS |
| 全名 | hydroxymethylbilane synthase |
| 背景介绍 | This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 39 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG58911 anti-HMBS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen stained with ARG58911 anti-HMBS antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
-
ARG58911 anti-HMBS antibody WB image
Western blot: 35 µg of K562 cell lysate (in RIPA buffer) stained with ARG58911 anti-HMBS antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
