ARG63020
anti-HLA E antibody [MEM-E/08]
anti-HLA E antibody [MEM-E/08] for Flow cytometry,Immunoprecipitation and Human
Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-E/08] recognizes HLA E |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IP |
| 特异性 | The clone MEM-E/08 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-E/08 |
| 同位型 | IgG1 |
| 靶点名称 | HLA E |
| 抗原 | Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide. |
| 偶联标记 | Un-conjugated |
| 別名 | MHC class I antigen E; QA1; EA2.1; HLA-6.2; EA1.2; MHC; HLA class I histocompatibility antigen, alpha chain E |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | FACS: LCL 721.221 (HLA-E transfected) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13747 Human HLA class I histocompatibility antigen, alpha chain E |
|---|---|
| 基因名称 | HLA-E |
| 全名 | major histocompatibility complex, class I, E |
| 背景介绍 | HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| 生物功能 | Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. [UniProt] |
| 研究领域 | Immune System antibody |
| 预测分子量 | 40 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG63020 anti-HLA E antibody [MEM-E/08] FACS image
Flow Cytometry: Separation of unstained (black) and stained (red) HLA-E transfected cells. Cells were stained with ARG63020 anti-HLA E antibody [MEM-E/08] at 0.33 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
HLA-E monoclonal antibodies recognize shared peptide sequences on classical HLA class Ia: relevance to human natural HLA antibodies.
ELISA / Human
