ARG41727

anti-HLA DMB antibody

anti-HLA DMB antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes HLA DMB
反应物种 Hu
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 HLA DMB
抗原物种 Human
抗原 Synthetic peptide corresponding to aa. 81-100 of Human HLA DMB. (NQKDTLMQRLRNGLQNCATH)
偶联标记 Un-conjugated
別名 RING7; D6S221E; HLA class II histocompatibility antigen, DM beta chain; MHC class II antigen DMB; Really interesting new gene 7 protein

应用说明

应用建议
应用 推荐稀释比
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 29 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Thimerosal and 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3109 Human HLA-DMB

Swiss-port # P28068 Human HLA class II histocompatibility antigen, DM beta chain

基因名称 HLA-DMB
全名 major histocompatibility complex, class II, DM beta
背景介绍 HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
生物功能 Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. [UniProt]
细胞定位 Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Localizes to late endocytic compartment. Associates with lysosome membranes. [UniProt]
预测分子量 29 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG41727 anti-HLA DMB antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue stained with ARG41727 anti-HLA DMB antibody.

  • ARG41727 anti-HLA DMB antibody WB image

    Western blot: 40 µg of Jurkat, Raji and HuT whole cell lysates stained with ARG41727 anti-HLA DMB antibody at 0.5 µg/ml dilution.