ARG53936
anti-HLA Class I antibody [MEM-147] (PE)
anti-HLA Class I antibody [MEM-147] (PE) for Flow cytometry and Human
Immune System antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [MEM-147] recognizes HLA Class I |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-147 |
| 同位型 | IgG1 |
| 靶点名称 | HLA Class I |
| 抗原 | PHA-activated peripheral blood lymphocytes |
| 偶联标记 | PE |
| 別名 | MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain |
|---|---|
| 基因名称 | HLA-A |
| 全名 | major histocompatibility complex, class I, A |
| 背景介绍 | HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. |
| 生物功能 | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
| 研究领域 | Immune System antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG53936 anti-HLA Class I antibody [MEM-147] (PE) FACS image
Flow Cytometry: Human peripheral blood stained with ARG53936 anti-HLA Class I antibody [MEM-147] (PE).
克隆号文献
The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex.
