ARG59691
anti-HIC1 antibody
anti-HIC1 antibody for Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes HIC1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HIC1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human HIC1. (NP_006488.2; NP_001091672.1, HLEGYPCPHLAYGEP) |
| 偶联标记 | Un-conjugated |
| 別名 | Zinc finger and BTB domain-containing protein 29; Hypermethylated in cancer 1 protein; ZNF901; ZBTB29; Hic-1; hic-1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14526 Human Hypermethylated in cancer 1 protein |
|---|---|
| 基因名称 | HIC1 |
| 全名 | hypermethylated in cancer 1 |
| 背景介绍 | This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010] |
| 生物功能 | Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from ACKR3, FGFBP1 and EFNA1. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1. Sumoylated on Lys-333 by a PIAS family member, which enhances interaction with MTA1, positively regulates transcriptional repression activity and is enhanced by HDAC4. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59691 anti-HIC1 antibody WB image
Western blot: HEK293 cells with mock transfection (left) or overexpressing Human HIC1 (right). The blots were stained with ARG59691 anti-HIC1 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG59691 anti-HIC1 antibody WB image
Western blot: 35 µg of A549 and Jurkat cell lysates (in RIPA buffer) stained with ARG59691 anti-HIC1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
