ARG57022
anti-HEXA antibody [20F1]
anti-HEXA antibody [20F1] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [20F1] recognizes HEXA |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 20F1 |
| 同位型 | IgG2a, lambda |
| 靶点名称 | HEXA |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 89-529 of Human HEXA. |
| 偶联标记 | Un-conjugated |
| 別名 | N-acetyl-beta-glucosaminidase subunit alpha; Beta-N-acetylhexosaminidase subunit alpha; EC 3.2.1.52; TSD; Beta-hexosaminidase subunit alpha; Hexosaminidase subunit A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HEXA |
| 全名 | hexosaminidase A (alpha polypeptide) |
| 背景介绍 | This gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I). [provided by RefSeq, Jul 2009] |
| 生物功能 | Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. [UniProt] |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | N-linked glycan at Asn-115 consists of Man(3)-GlcNAc(2). |
检测图片 (3) Click the Picture to Zoom In
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ARG57022 anti-HEXA antibody [20F1] FACS image
Flow Cytometry: A549 cell line stained with ARG57022 anti-HEXA antibody [20F1] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57022 anti-HEXA antibody [20F1] WB image
Western blot: 40 µg of MCF7 cell lysate stained with ARG57022 anti-HEXA antibody [20F1] at 1:3000.
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ARG57022 anti-HEXA antibody [20F1] WB image
Western blot: 10 µg of 1) 293T cell lysate, 2) HEXA Transfected 293T cell lysate stained with ARG57022 anti-HEXA antibody [20F1] at 1:3000.
