ARG43091
anti-HECTD3 antibody
anti-HECTD3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HECTD3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HECTD3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human HECTD3. (HYASAKVCEEKLRYAAYNCVAIDTDMSPWEE) |
| 偶联标记 | Un-conjugated |
| 別名 | E3 ubiquitin-protein ligase HECTD3; EC 6.3.2.-; HECT domain-containing protein 3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Caco-2, Rat brain and Mouse brain | ||||||||
| 实际分子量 | ~ 97 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q3U487 Mouse E3 ubiquitin-protein ligase HECTD3 Swiss-port # Q5T447 Human E3 ubiquitin-protein ligase HECTD3 |
|---|---|
| 基因名称 | HECTD3 |
| 全名 | HECT domain containing E3 ubiquitin protein ligase 3 |
| 背景介绍 | The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012] |
| 生物功能 | E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm, perinuclear region. [UniProt] |
| 预测分子量 | 97 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG43091 anti-HECTD3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43091 anti-HECTD3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43091 anti-HECTD3 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Caco-2, Rat brain and Mouse brain lysates stained with ARG43091 anti-HECTD3 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43091 anti-HECTD3 antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG43091 anti-HECTD3 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43091 anti-HECTD3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43091 anti-HECTD3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43091 anti-HECTD3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43091 anti-HECTD3 antibody at 1 µg/ml dilution, overnight at 4°C.
