ARG66283

anti-HDGF antibody [SQab1746]

anti-HDGF antibody [SQab1746] for ELISA,Flow cytometry,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

Cancer; Growth factor

概述

产品描述 Mouse Monoclonal antibody [SQab1746] recognizes HDGF
反应物种 Hu, Ms, Rat
应用 ELISA, FACS, ICC/IF, IHC, WB
宿主 Mouse
克隆 Monoclonal
克隆号 SQab1746
同位型 IgG1
靶点名称 HDGF
抗原物种 Human
抗原 Recombinant Human HDGF protein.
偶联标记 Un-conjugated
別名 HMG1L2; HMG-1L2; HDGF; High mobility group protein 1-like 2; Hepatoma-derived growth factor

应用说明

应用建议
应用 推荐稀释比
ELISA1:3000 - 1:10000
FACS1:400 - 1:1000
ICC/IF1:400 - 1:1000
IHCAssay-dependent
WB1:2500 - 1:5000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂 0.01% Thimerosal
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 114499 Rat HDGF

GeneID: 15191 Mouse HDGF

GeneID: 3068 Human HDGF

基因名称 HDGF
全名 hepatoma-derived growth factor
背景介绍 HDGF is a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]
生物功能 HDGF Isoform 1: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618).

Isoform 2: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719).

Isoform 3: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719). [UniProt]
产品亮点 Related products:
HDGF antibodies; HDGF ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
The most sensitive human HDGF ELISA kit
The role of HDGF in tumor angiogenesis
研究领域 Cancer; Growth factor
预测分子量 27 kDa
翻译后修饰 Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. [UniProt]

检测图片 (7) Click the Picture to Zoom In

  • ARG66283 anti-HDGF antibody [SQab1746] ICC/IF image

    Immunofluorescence: Cells (A549 and MCF-7) were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66283 anti-HDGF antibody [SQab1746] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).

  • ARG66283 anti-HDGF antibody [SQab1746] FACS image

    Flow Cytometry: 293T cells stained with ARG66283 anti-HDGF antibody [SQab1746] at 1 µg/ml (red) and without antibody control (black).

  • ARG66283 anti-HDGF antibody [SQab1746] WB image

    Western blot: 15 µg of 293T cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.

  • ARG66283 anti-HDGF antibody [SQab1746] ELISA image

    ELISA: Titration curve of ARG66283 anti-HDGF antibody [SQab1746] in ELISA. Red: HDGF; Black: BSA (negative control).

  • ARG66283 anti-HDGF antibody [SQab1746] WB image

    Western blot: 20 µg of HuH7 cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.

  • ARG66283 anti-HDGF antibody [SQab1746] WB image

    Western blot: 20 µg of 3T3 cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.

  • ARG66283 anti-HDGF antibody [SQab1746] WB image

    Western blot: 50 µg Mouse liver and Rat liver lysates stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.