ARG52324
anti-HDAC2 phospho (Ser394) antibody
anti-HDAC2 phospho (Ser394) antibody for Western blot and Mouse
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HDAC2 phospho (Ser394) |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, Rat, Bov, Chk, Gpig, NHuPrm, Sheep |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HDAC2 |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Histone deacetylase 2; EC 3.5.1.98; HD2; YAF1; RPD3 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~55k HDAC2 protein phosphorylated at Ser394 in Western blots. Immunolabeling is completely blocked by λ-Phosphatase treatment (30 minutes, 800units/1mg protein). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HDAC2 |
| 全名 | histone deacetylase 2 |
| 背景介绍 | Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of ser394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of ser422 and ser424 (EOM et al, 2011). |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. S-Nitrosylation interferes with its interaction with MTA1 (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG52324 anti-HDAC2 phospho (Ser394) antibody WB image
Western blot: Mouse heart lysate showing specific immunolabeling of the ~55k HDAC2 protein phosphorylated at Ser 394 (control) stained with ARG52324 anti-HDAC2 phospho (Ser394) antibody.
Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase).
The blot is identical to the control except that the lysate was incubated in λ-Ptase (800 units/1mg protein for 30 min).
The immunolabeling is completely eliminated by treatment with λ-Ptase.
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