ARG55651

anti-HDAC2 antibody

anti-HDAC2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes HDAC2
反应物种 Hu, Ms, Rat
应用 IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 HDAC2
抗原物种 Human
抗原 Synthetic peptide of Human HDAC2 (NP_001518.3)
偶联标记 Un-conjugated
別名 Histone deacetylase 2; EC 3.5.1.98; HD2; YAF1; RPD3

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:200
IP1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HeLa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 15182 Mouse HDAC2

GeneID: 3066 Human HDAC2

Swiss-port # P70288 Mouse Histone deacetylase 2

Swiss-port # Q92769 Human Histone deacetylase 2

基因名称 HDAC2
全名 histone deacetylase 2
背景介绍 This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
生物功能 Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. [UniProt]
预测分子量 55 kDa
翻译后修饰 S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. S-Nitrosylation interferes with its interaction with MTA1 (By similarity).

检测图片 (3) Click the Picture to Zoom In

  • ARG55651 anti-HDAC2 antibody WB image

    Western blot: 25 µg of HeLa cell lysate stained with ARG55651 anti-HDAC2 antibody at 1:1000 dilution.

  • ARG55651 anti-HDAC2 antibody IP image

    Immunoprecipitation: 200 µg extracts of K562 cells immunoprecipitated and stained with ARG55651 anti-HDAC2 antibody at 1:1000 dilution.

  • ARG55651 anti-HDAC2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon carcinoma stained with ARG55651 anti-HDAC2 antibody at 1:100 dilution (40x lens).