ARG55233

Name: anti-HDAC2 antibody
Brand: arigo Biolaboratories
SKU: ARG55233
Price: 399 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-HDAC2 antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

50 μl

279

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes HDAC2
反应物种	Hu, Ms
预测物种	Chk
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	HDAC2
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 410-439 (Center) of Human HDAC2.
偶联标记	Un-conjugated
別名	Histone deacetylase 2; EC 3.5.1.98; HD2; YAF1; RPD3

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
ICC/IF	1:10 - 1:50
IHC-P	1:10 - 1:50
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A and immunogen peptide.
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 15182 Mouse HDAC2 GeneID: 3066 Human HDAC2 Swiss-port # P70288 Mouse Histone deacetylase 2 Swiss-port # Q92769 Human Histone deacetylase 2
基因名称	HDAC2
全名	histone deacetylase 2
背景介绍	This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
生物功能	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. [UniProt]
细胞定位	Nucleus. Cytoplasm
研究领域	Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
预测分子量	55 kDa
翻译后修饰	S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. S-Nitrosylation interferes with its interaction with MTA1 (By similarity).

检测图片 (4) Click the Picture to Zoom In

ARG55233 anti-HDAC2 antibody ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55233 anti-HDAC2 antibody (orange) at 1:10 dilution.

Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
ARG55233 anti-HDAC2 antibody WB image

Western blot: 30 μg of HeLa, Jurkat, HEK293T, NIH-3T3 and Mouse brain lysates stained with ARG55233 anti-HDAC2 antibody at 1:500 dilution.
ARG55233 anti-HDAC2 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG55233 anti-HDAC2 antibody.
ARG55233 anti-HDAC2 antibody FACS image

Flow Cytometry: K562 cells stained with ARG55233 anti-HDAC2 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.