ARG10064

anti-HCV Core antigen / HCcAg antibody [B2]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Mouse Monoclonal antibody [B2] recognizes Hepatitis C virus core antigen
反应物种	HCV
应用	ELISA, ICC/IF, WB
特异性	No cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
宿主	Mouse
克隆	Monoclonal
克隆号	B2
同位型	IgG1, kappa
靶点名称	HCV Core antigen / HCcAg
抗原物种	HCV
抗原	Synthetic peptides derived from HCV core protein
偶联标记	Un-conjugated

应用说明

应用说明	ELISA: The mAb reacts with human hepatitis C virus core protein, and doesn’t react with HCV non-structure protein. Western Blot: The mAb, when used at concentration of 0.1-0.5μg/mL, will allow visualization of 0.1 μg/lane of recombinant core protein C + envelope protein M, 0.5μg /lane synthetic core protein C, and 0.1μg /lane recombinant chimeric HCV polyprotein (552 aa, around 60 kDa). The mAb works on blots transferred from both reducing and non-reducing PAGE gel. The mAb recognizes in-vitro translated HCV core protein (around 20 kDa) when used at concentration of 1 μg/mL. Immunofluorescent analysis: The mAb recognizes HCV core in HCV core expressing plasmid transfected cells at 1:200 dilution. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

ELISA: The mAb reacts with human hepatitis C virus core protein, and doesn’t react with HCV non-structure protein.

Western Blot: The mAb, when used at concentration of 0.1-0.5μg/mL, will allow visualization of 0.1 μg/lane of recombinant core protein C + envelope protein M, 0.5μg /lane synthetic core protein C, and 0.1μg /lane recombinant chimeric HCV polyprotein (552 aa, around 60 kDa). The mAb works on blots transferred from both reducing and non-reducing PAGE gel. The mAb recognizes in-vitro translated HCV core protein (around 20 kDa) when used at concentration of 1 μg/mL.

Immunofluorescent analysis: The mAb recognizes HCV core in HCV core expressing plasmid transfected cells at 1:200 dilution.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Protein G affinity purified
缓冲液	0.01M PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 951475 HCV HCVgp1
背景介绍	Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of Core Protein (Capsid Protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.
研究领域	Microbiology and Infectious Disease antibody

检测图片 (3) Click the Picture to Zoom In

ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] ICC/IF image

Immunofluorescence: Cells stained with ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] (green).
ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] WB image

Western blot: HCV Core protein expressing plasmid transfected 293 cells stained with ARG10064 anti-HCV Core antigen / HCcAg antibody [B2].
ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] WB image

Western blot: HCV Core protein stable expressed Huh7 cells stained with ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] and anti-beta actin antibody.