ARG22223
anti-HCN2 antibody [S71-37]
anti-HCN2 antibody [S71-37] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [S71-37] recognizes HCN2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 特异性 | Detects ~95kDa. No cross-reactivity against HCN1. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S71-37 |
| 同位型 | IgG1 |
| 靶点名称 | HCN2 |
| 抗原物种 | Rat |
| 抗原 | Fusion protein around aa. 761-863 of Rat HCN2 |
| 偶联标记 | Un-conjugated |
| 別名 | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; HAC-1; BCNG2; Brain cyclic nucleotide-gated channel 2; BCNG-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Hcn2 |
| 全名 | hyperpolarization activated cyclic nucleotide-gated potassium channel 2 |
| 背景介绍 | Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM, Jul 2010] |
| 生物功能 | Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity). [UniProt] |
| 细胞定位 | Membrane |
| 预测分子量 | 97 kDa |
| 翻译后修饰 | Phosphorylation at Ser-668 by PRKG2 shifts the voltage-dependence to more negative voltages, hence counteracting the stimulatory effect of cGMP on gating. |
检测图片 (2) Click the Picture to Zoom In
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ARG22223 anti-HCN2 antibody [S71-37] WB image
Western blot: Rat brain membrane lysate stained with ARG22223 anti-HCN2 Antibody [S71-37] at 1:1000 dilution.
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ARG22223 anti-HCN2 antibody [S71-37] IHC-Fr image
Immunohistochemistry: 10% Formalin (12-24 hours at RT) fixed Mouse frozen brain section stained with ARG22223 anti-HCN2 Antibody [S71-37] (brown) at 1:1000 dilution (1 hour). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500uL for 5 minutes at RT.
