ARG66474
anti-HAND1 antibody
anti-HAND1 antibody for ICC/IF,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HAND1 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Bov, Pig, Rb, Sheep |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HAND1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide within the C-terminal region of Human HAND1. |
| 偶联标记 | Un-conjugated |
| 別名 | Class A basic helix-loop-helix protein 27; Thing1; bHLHa27; eHAND; Hxt; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; Heart- and neural crest derivatives-expressed protein 1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O96004 Human Heart- and neural crest derivatives-expressed protein 1 Swiss-port # P97832 Rat Heart- and neural crest derivatives-expressed protein 1 |
|---|---|
| 基因名称 | HAND1 |
| 全名 | heart and neural crest derivatives expressed 1 |
| 背景介绍 | The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity). [UniProt] |
| 细胞定位 | Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity). [UniProt] |
| 预测分子量 | 24 kDa |
| 翻译后修饰 | Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG66474 anti-HAND1 antibody ICC/IF image
Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at RT. Cells were stained with ARG66474 anti-HAND1 antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber.
-
ARG66474 anti-HAND1 antibody WB image
Western blot: HeLa and H9C2 whole cell lysates stained with ARG66474 anti-HAND1 antibody.
