ARG64362

anti-HADH / HADHSC antibody

anti-HADH / HADHSC antibody for Western blot and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes HADH / HADHSC
反应物种 Hu
预测物种 Ms, Rat, Dog
应用 WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 HADH / HADHSC
抗原物种 Human
抗原 C-YERGDASKEDID
偶联标记 Un-conjugated
別名 Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; HAD; EC 1.1.1.35; HADHSC; HHF4; HCDH; SCHAD; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HADH1; MSCHAD

应用说明

应用建议
应用 推荐稀释比
WB0.01 - 0.03 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3033 Human HADH

Swiss-port # Q16836 Human Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial

背景介绍 This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
研究领域 Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 34 kDa
翻译后修饰 Succinylation at Lys-81, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).

检测图片 (2) Click the Picture to Zoom In

  • ARG64362 anti-HADH / HADHSC antibody WB image

    Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG64362 anti-HADH / HADHSC antibody at 0.02 µg/ml dilution.

  • ARG64362 anti-HADH / HADHSC antibody WB image

    Western Blot: HEK293 overexpressing HADH (RC201752) with C-terminal tag (DYKDDDDK) and stained with anti-DYKDDDDK in the left panel and with ARG64362 anti-HADH / HADHSC antibody in the right panel (mock transfection in first and last lanes)