ARG41054
anti-HACE1 antibody
anti-HACE1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HACE1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HACE1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-100 of Human HACE1 (NP_065822.2). |
| 偶联标记 | Un-conjugated |
| 別名 | HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; EC 6.3.2.-; E3 ubiquitin-protein ligase HACE1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293T | ||||||
| 实际分子量 | 102 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HACE1 |
| 全名 | HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 |
| 生物功能 | E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. [UniProt] |
| 细胞定位 | Golgi apparatus, Golgi stack membrane. Cytoplasm. Endoplasmic reticulum. Note=A significant portion localizes to the endoplasmic reticulum. Targeted to Golgi membrane via its interaction with Rab proteins. [UniProt] |
| 预测分子量 | 102 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG41054 anti-HACE1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG41054 anti-HACE1 antibody at 1:100 dilution.
-
ARG41054 anti-HACE1 antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG41054 anti-HACE1 antibody at 1:1000 dilution.
