ARG66389
anti-HA tag antibody [SQab18117]
anti-HA tag antibody [SQab18117] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab18117] recognizes HA tag |
|---|---|
| 反应物种 | Other |
| 应用 | FACS, ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab18117 |
| 同位型 | IgG |
| 靶点名称 | HA tag |
| 抗原物种 | Others |
| 抗原 | YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (6) Click the Picture to Zoom In
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ARG66389 anti-HA tag antibody [SQab18117] ICC/IF image
Immunofluorescence: 293 cells transfected with N-terminal HA tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:2000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.
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ARG66389 anti-HA tag antibody [SQab18117] FACS image
Flow Cytometry: 293 cells transfected with N-terminal (green) or C-terminal (red) HA tagged gene. The cells were then stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:2000 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66389 anti-HA tag antibody [SQab18117] WB image
Western blot: 1) 1 µg of 293 cells transfected with C-terminal HA tagged gene and stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:20000 dilution. 2) 10 µg of 293 cells transfected with N-terminal HA tagged gene and stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:1000 dilution. 3) 10 µg of 293 cells (without any transfection) stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:400 dilution.
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ARG66389 anti-HA tag antibody [SQab18117] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysate (transfected with N-terminal HA tagged gene) was immunoprecipitated (1:10 dilution) and stained with ARG66389 anti-HA tag antibody [SQab18117].
Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with N-terminal HA tagged gene)
Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with N-terminal HA tagged gene)
Lane 3: 293 whole cell lysate (transfected with N-terminal HA tagged gene), 10 µg (input) -
ARG66389 anti-HA tag antibody [SQab18117] ICC/IF image
Immunofluorescence: 293 cells transfected with C-terminal HA tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:2000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.
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ARG66389 anti-HA tag antibody [SQab18117] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysate (transfected with C-terminal HA tagged gene) was immunoprecipitated (1:10 dilution) and stained with ARG66389 anti-HA tag antibody [SQab18117].
Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with C-terminal HA tagged gene)
Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with C-terminal HA tagged gene)
Lane 3: 293 whole cell lysate (transfected with C-terminal HA tagged gene), 10 µg (input)
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anti-HA tag antibody [SQab18117]
Application：IHC
Sample：Frozen Mouse brain tissue section
Sample Preparation Method：PBS and 4% PFA perfusion
Fixation Buffer：4% PFA
Fixation Time：overnight
Antigen Retrieval Buffer：Citrate pH 6.0
Permeabilization Buffer：0.3% PBST (Triton x-100)
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
