ARG22536
anti-Granzyme A antibody [GA6]
anti-Granzyme A antibody [GA6] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [GA6] recognizes Granzyme A This antibody recognizes Granzyme A, a ~60 kDa disulphide-linked homodimeric protein of two 262 amino acid chains, expressed in cytoplasmic granules of cytotoxic lymphocytes and NK cells.Granzyme A is involved in the induction of apoptosis via its activity as a serine protease, but this would seem to be subsidiary to the role of Granzyme B. Granzyme A deficient mice are indistinguishable from normal animals in their response to infection.Granzyme A has been proposed as a potential biomarker for patients with active tuberculosis with significantly lower levels present in the plasma of patients with the active form of the disease compared to patients with latent infection (Guggino et al. 2015) |
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| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GA6 |
| 同位型 | IgG1 |
| 靶点名称 | Granzyme A |
| 抗原物种 | Human |
| 抗原 | Recombinant human Granzyme A. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.4.21.78; in-1; CTLA3; CTL tryptase; Granzyme A; Hanukkah factor; Cytotoxic T-lymphocyte proteinase 1; H factor; HFSP; HF; Granzyme-1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | GZMA |
| 全名 | granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) |
| 背景介绍 | Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008] |
| 生物功能 | Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA. [UniProt] |
| 预测分子量 | 29 kDa |
