ARG45544
anti-Glutamyl Prolyl tRNA synthetase / PARS antibody
anti-Glutamyl Prolyl tRNA synthetase / PARS antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Glutamyl Prolyl tRNA synthetase / PARS |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Glutamyl Prolyl tRNA synthetase / PARS |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human Glutamyl Prolyl tRNA synthetase / PARS. |
| 偶联标记 | Un-conjugated |
| 別名 | EPRS1; Glutamyl-Prolyl-TRNA Synthetase 1; PARS; GLUPRORS; EARS; EPRS; QARS; QPRS; Bifunctional Glutamate/Proline--TRNA Ligase; Cell Proliferation-Inducing Gene 32 Protein; Bifunctional Aminoacyl-TRNA Synthetase; Glutamatyl-Prolyl-TRNA Synthetase; Glutamate TRNA Ligase; Proliferation-Inducing Gene 32 Protein; Proliferation-Inducing Protein 32; Glutamyl-Prolyl-TRNA Synthetase; Glutaminyl-TRNA Synthetase; Prolyl-TRNA Synthetase; Proline TRNA Ligase; Proline-TRNA Ligase; EC 6.1.1.15; EC 6.1.1.18; EC 6.1.1; HLD15; PIG32; GLNS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 170 - 180 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P07814 Human Bifunctional glutamate/proline--tRNA ligase Swiss-port # Q8CGC7 Mouse Bifunctional glutamate/proline--tRNA ligase |
|---|---|
| 基因名称 | EPRS1 |
| 全名 | Glutamyl-Prolyl-TRNA Synthetase 1 |
| 背景介绍 | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro). [UniProt] |
| 细胞定位 | Cytoplasm; Membrane. [UniProt] |
| 预测分子量 | 171 kDa |
| 翻译后修饰 | Acetylation; Methylation; Phosphoprotein. [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody IHC-P image
Immunohistochemistry: Human lung cancer stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 2 μg/ml dilution.
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 5 μg/ml dilution.
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody WB image
Western blot: Hek293 stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 0.5 μg/ml dilution.
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody WB image
Western blot: Rat pancreas stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 0.5 μg/ml dilution.
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody FACS image
Flow Cytometry: THP-1 stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 1 µg/10^6 cells dilution.
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 2 μg/ml dilution.
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 2 μg/ml dilution.
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ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody WB image
Western blot: Mouse pancreas stained with ARG45544 anti-Glutamyl Prolyl tRNA synthetase / PARS antibody at 0.5 μg/ml dilution.
