ARG52319
anti-GluR2 subunit phospho (Ser880) antibody
anti-GluR2 subunit phospho (Ser880) antibody for Western blot and Rat
Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GluR2 subunit phospho (Ser880) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, NHuPrm, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GluR2 subunit |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser880 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | AMPA-selective glutamate receptor 2; GluA2; GluR-K2; Glutamate receptor ionotropic, AMPA 2; GluR-2; HBGR2; GLUR2; GluR-B; Glutamate receptor 2; GLURB |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | Specific for the ~100k GluR2 protein phosphorylated at Ser880. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GRIA2 |
| 全名 | glutamate receptor, ionotropic, AMPA 2 |
| 背景介绍 | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The number of GluR2 subunits in the AMPA receptor complex affects the Ca2+ permeability, rectification and single-channel conductance of AMPA receptors. Ser880 has been identified as the PKC phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1 (Matsuda et al., 1999) |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 99 kDa |
| 翻译后修饰 | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). |
检测图片 (1) Click the Picture to Zoom In
-
ARG52319 anti-GluR2 subunit phospho (Ser880) antibody WB image
Western blot: Rat brain homogenate showing specific immunolabeling of the ~100 kDa GluR2 protein phosphorylated at Ser880 (control) stained with ARG52319 anti-GluR2 subunit phospho (Ser880) antibody. Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
