ARG10605
anti-GluR2 antibody
anti-GluR2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GluR2 |
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| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GluR2 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein around aa. 25-360 of Human GluR2 |
| 偶联标记 | Un-conjugated |
| 別名 | AMPA-selective glutamate receptor 2; GluA2; GluR-K2; Glutamate receptor ionotropic, AMPA 2; GluR-2; HBGR2; GLUR2; GluR-B; Glutamate receptor 2; GLURB |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue sections in 10 mM Citrate buffer (pH 6.0) for 20 min * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Rat brain, C6 and Mouse brain | ||||||||||
| 实际分子量 | ~ 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2.5% BSA. |
| 抗菌剂 | 0.025% Sodium azide |
| 稳定剂 | 2.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GRIA2 |
| 全名 | glutamate receptor, ionotropic, AMPA 2 |
| 背景介绍 | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. [UniProt] |
| 产品亮点 | Related products: GluR2 antibodies; Related news: Viral-like capsids, new trans-synaptic mRNA transport mechanism |
| 预测分子量 | 99 kDa |
| 翻译后修饰 | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). |
检测图片 (6) Click the Picture to Zoom In
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ARG10605 anti-GluR2 antibody ICC/IF image
Immunofluorescence: Mouse brain tissues stained with ARG10605 anti-GluR2 antibody at 2μg/mL dilution.
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ARG10605 anti-GluR2 antibody IHC-P image
Immunohistochemistry: Mouse brain tissue stained with ARG10605 anti-GluR2 antibody at 1μg/mL dilution.
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ARG10605 anti-GluR2 antibody WB image
Western blot: 1) Rat brain, and 2) Mouse brain lysate stained with ARG10605 anti-GluR2 antibody at 0.5μg/mL dilution.
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ARG10605 anti-GluR2 antibody FACS image
Flow Cytometry: U-87MG stained with ARG10605 anti-GluR2 antibody at 1μg/1x10^6 cells dilution.
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ARG10605 anti-GluR2 antibody WB image
Western blot: Rat brain, C6 and Mouse brain lysates stained with ARG10605 anti-GluR2 antibody at 0.5μg/mL dilution.
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ARG10605 anti-GluR2 antibody IHC-P image
Immunohistochemistry: Rat brain tissue stained with ARG10605 anti-GluR2 antibody at 1μg/mL dilution.
