ARG45904
anti-Germinal Center Kinase / GCK antibody
anti-Germinal Center Kinase / GCK antibody for Western blot and Human,Mouse,Rat,Monkey
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Germinal Center Kinase / GCK |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mk |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Germinal Center Kinase / GCK |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human Germinal Center Kinase / GCK. |
| 偶联标记 | Un-conjugated |
| 別名 | MAP4K2; GCK; RAB8IP; Mitogen-activated protein kinase kinase kinase kinase 2; EC 2.7.11.1; B lymphocyte serine/threonine-protein kinase; Germinal center kinase; GC kinase; MAPK/ERK kinase kinase kinase 2; MEK kinase kinase 2; MEKKK 2; Rab8-interacting protein |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | 97 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q12851 Human Mitogen-activated protein kinase kinase kinase kinase 2 Swiss-port # Q61161 Mouse Mitogen-activated protein kinase kinase kinase kinase 2 |
|---|---|
| 基因名称 | MAP4K2 |
| 全名 | Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 |
| 背景介绍 | The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
| 生物功能 | Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Also mediates the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway. [UniProt] |
| 细胞定位 | Cell membrane; Cytoplasm; Golgi apparatus; Membrane. [UniProt] |
| 预测分子量 | 92 kDa |
| 翻译后修饰 | Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
-
ARG45904 anti-Germinal Center Kinase / GCK antibody WB image
Western blot: Hela stained with ARG45904 anti-Germinal Center Kinase / GCK antibody at 0.5 μg/ml dilution.
-
ARG45904 anti-Germinal Center Kinase / GCK antibody WB image
Western blot: COS-7 stained with ARG45904 anti-Germinal Center Kinase / GCK antibody at 0.5 μg/ml dilution.
-
ARG45904 anti-Germinal Center Kinase / GCK antibody WB image
Western blot: Rat brain stained with ARG45904 anti-Germinal Center Kinase / GCK antibody at 0.5 μg/ml dilution.
-
ARG45904 anti-Germinal Center Kinase / GCK antibody WB image
Western blot: 3T3-L1 stained with ARG45904 anti-Germinal Center Kinase / GCK antibody at 0.5 μg/ml dilution.
