ARG56927
anti-Geminin antibody [2H7]
anti-Geminin antibody [2H7] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [2H7] recognizes Geminin |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2H7 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Geminin |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-209 of Human Geminin. |
| 偶联标记 | Un-conjugated |
| 別名 | Geminin; Gem |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GMNN |
| 全名 | geminin, DNA replication inhibitor |
| 背景介绍 | This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011] |
| 生物功能 | Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control. [UniProt] |
| 预测分子量 | 24 kDa |
| 翻译后修饰 | Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity. |
检测图片 (1) Click the Picture to Zoom In
-
ARG56927 anti-Geminin antibody [2H7] WB image
Western blot: 50 µg of 293T cell lysates stained with ARG56927 anti-Geminin antibody [2H7] at 1:500.
