ARG45589

anti-Gelsolin antibody

anti-Gelsolin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes Gelsolin
反应物种 Hu, Ms, Rat, Mk
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Gelsolin
抗原物种 Human
抗原 Recombinant protein containing to human Gelsolin.
偶联标记 Un-conjugated
別名 GSN; gelsolin; ADF; Actin-depolymerizing factor; Brevin; AGEL; Gelsolin

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF2 μg/ml
IHC-P0.5-1 μg/ml
WB0.1-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 86 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 227753 Mouse GSN

GeneID: 2934 Human GSN

GeneID: 296654 Rat GSN

基因名称 GSN
全名 gelsolin
背景介绍 The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能 Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. [UniProt]
细胞定位 Isoform 2: Cytoplasm, cytoskeleton.. [UniProt]
预测分子量 86 kDa
翻译后修饰 Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45589 anti-Gelsolin antibody WB image

    Western blot: Hela and THP-1 stained with ARG45589 anti-Gelsolin antibody at 0.5 μg/ml dilution.

  • ARG45589 anti-Gelsolin antibody WB image

    Western blot: RAW264.7 stained with ARG45589 anti-Gelsolin antibody at 0.5 μg/ml dilution.

  • ARG45589 anti-Gelsolin antibody IHC-P image

    Immunohistochemistry: Rat spleen stained with ARG45589 anti-Gelsolin antibody at 1 μg/ml dilution.

  • ARG45589 anti-Gelsolin antibody WB image

    Western blot: COS-7 stained with ARG45589 anti-Gelsolin antibody at 0.5 μg/ml dilution.