ARG53062
anti-Galectin 1 antibody [SP247]
anti-Galectin 1 antibody [SP247] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody
概述
| 产品描述 | Rabbit Monoclonal antibody [SP247] recognizes Galectin 1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SP247 |
| 同位型 | IgG |
| 靶点名称 | Galectin 1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide near the C-terminus of the Human Galectin 1 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | HBL; Gal-1; GAL1; HPL; Lectin galactoside-binding soluble 1; Galectin-1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; GBP; Beta-galactoside-binding lectin L-14-I; Putative MAPK-activating protein PM12; 14 kDa lectin; Galaptin; S-Lac lectin 1; HLBP14 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubation Time: 10 at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Placenta, Tonsil, NIH/3T3 Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by protein A/G |
| 缓冲液 | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| 抗菌剂 | < 0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Galectin-1 (gal-1) is expressed intracellularly and extracellularly by various normal and pathological tissues. It shows a wide range of biological activity such as apoptosis, cell proliferation and cell differentiation. Gal-1 is overexpressed in endothelial cells of different human tumors, tumor cells, and extracellular matrix. It binds its ligands such as beta-galactoside and a wide array of complex carbohydrates to regulate immune responses. |
| 细胞定位 | Cytoplasm, Extracellular Matrix, Nucleus |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody |
| 预测分子量 | 15 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG53062 anti-Galectin 1 antibody [SP247] WB image
Western blot: 30 µg of A549 cell lysate stained with ARG53062 anti-Galectin 1 antibody [SP247] at 1:100 dilution.
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ARG53062 anti-Galectin 1 antibody [SP247] IHC-P image
Immunohistochemistry: Human Placenta stained with ARG53062 anti-Galectin 1 antibody [SP247].
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ARG53062 anti-Galectin 1 antibody [SP247] WB image
Western blot: NIH/3T3 cell lysate stained with ARG53062 anti-Galectin 1 antibody [SP247].
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ARG53062 anti-Galectin 1 antibody [SP247] WB image
Western blot: 30 µg of Mouse kidney lysate stained with ARG53062 anti-Galectin 1 antibody [SP247] at 1:100 dilution.
客户反馈
Good
anti-Galectin 1 antibody [SP247]
Application：WB
Sample：Rat kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Galectin 1 antibody [SP247]
Application：WB
Sample：Mouse kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
