ARG45894

anti-GTF2A2 antibody

anti-GTF2A2 antibody for Western blot,Flow cytometry and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes GTF2A2
反应物种 Hu, Ms, Rat
应用 FACS, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GTF2A2
抗原物种 Human
抗原 Recombinant protein containing to human GTF2A2.
偶联标记 Un-conjugated
別名 GTF2A2; TF2A2; Transcription initiation factor IIA subunit 2; General transcription factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; Transcription initiation factor IIA gamma chain; TFIIA-gamma

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 17 kDa

属性

形式 Liquid
纯化 Affinity chromatography purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 235459 Mouse GTF2A2

GeneID: 2958 Human GTF2A2

GeneID: 83828 Rat GTF2A2

基因名称 GTF2A2
全名 General Transcription Factor IIA Subunit 2
背景介绍 Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008]
生物功能 TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 12 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG45894 anti-GTF2A2 antibody FACS image

    Flow Cytometry: U251 stained with ARG45894 anti-GTF2A2 antibody at 1 µg/10^6 cells dilution.

  • ARG45894 anti-GTF2A2 antibody WB image

    Western blot: Rat testis stained with ARG45894 anti-GTF2A2 antibody at 0.5 μg/ml dilution.

  • ARG45894 anti-GTF2A2 antibody WB image

    Western blot: Mouse testis stained with ARG45894 anti-GTF2A2 antibody at 0.5 μg/ml dilution.