ARG63994
anti-GRP94 antibody
anti-GRP94 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GRP94 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog, Pig, Zfsh |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GRP94 |
| 抗原物种 | Human |
| 抗原 | C-KEGVKFDESEKTKE |
| 偶联标记 | Un-conjugated |
| 別名 | Tumor rejection antigen 1; gp96 homolog; Endoplasmin; 94 kDa glucose-regulated protein; HEL-S-125m; GP96; GRP94; TRA1; HEL35; GRP-94; Heat shock protein 90 kDa beta member 1; ECGP |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. [provided by RefSeq, Aug 2012] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 92 kDa |
检测图片 (8) Click the Picture to Zoom In
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ARG63994 anti-GRP94 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63994 anti-GRP94 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63994 anti-GRP94 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63994 anti-GRP94 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63994 anti-GRP94 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63994 anti-GRP94 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63994 anti-GRP94 antibody WB image
Western blot: 35 µg of U2OS (A), HEK293 (B), HeLa (C) and A549 (D) cell lysates (in RIPA buffer) stained with ARG63994 anti-GRP94 antibody at 0.1 µg/ml (A-C) and 0.3 µg/ml (D) dilutions and incubated at RT for 1 hour.
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ARG63994 anti-GRP94 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG63994 anti-GRP94 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63994 anti-GRP94 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63994 anti-GRP94 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63994 anti-GRP94 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63994 anti-GRP94 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63994 anti-GRP94 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG63994 anti-GRP94 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
