ARG22214
anti-GRP94 antibody [9G10]
anti-GRP94 antibody [9G10] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Guinea pig,Hamster,Horse,Monkey,Pig,Rabbit,Sheep,Xenopus laevis
概述
| 产品描述 | Rat Monoclonal antibody [9G10] recognizes GRP94 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Chk, Dog, Gpig, Hm, Hrs, Mk, Pig, Rb, Sheep, Xenopus laevis |
| 应用 | FACS, ICC/IF, IP, WB |
| 特异性 | Detects ~98kDa. Does not detect human Hsp90, Grp74, or GrpE from E.coli. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | 9G10 |
| 同位型 | IgG2a |
| 靶点名称 | GRP94 |
| 抗原物种 | Chicken |
| 抗原 | Purified GRP94 isolated from chicken oviducts |
| 偶联标记 | Un-conjugated |
| 別名 | Tumor rejection antigen 1; gp96 homolog; Endoplasmin; 94 kDa glucose-regulated protein; HEL-S-125m; GP96; GRP94; TRA1; HEL35; GRP-94; Heat shock protein 90 kDa beta member 1; ECGP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSP90B1 |
| 全名 | heat shock protein 90kDa beta (Grp94), member 1 |
| 背景介绍 | This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012] |
| 生物功能 | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. [UniProt] |
| 细胞定位 | Endoplasmic Reticulum, Endoplasmic reticulum lumen, Melanosome |
| 预测分子量 | 92 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG22214 anti-GRP94 antibody [9G10] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22214 anti-GRP94 antibody [9G10] (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22214 anti-GRP94 antibody [9G10] WB image
Western blot: Human HeLa cell lysates stained with ARG22214 anti-GRP94 antibody [9G10] at 1:1000 dilution.
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ARG22214 anti-GRP94 antibody [9G10] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22214 anti-GRP94 antibody [9G10] (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
