ARG57042
anti-GRP94 antibody [94B9]
anti-GRP94 antibody [94B9] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [94B9] recognizes GRP94 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 94B9 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | GRP94 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 22-803 of Human GPR94. |
| 偶联标记 | Un-conjugated |
| 別名 | Tumor rejection antigen 1; gp96 homolog; Endoplasmin; 94 kDa glucose-regulated protein; HEL-S-125m; GP96; GRP94; TRA1; HEL35; GRP-94; Heat shock protein 90 kDa beta member 1; ECGP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSP90B1 |
| 全名 | heat shock protein 90kDa beta (Grp94), member 1 |
| 背景介绍 | This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012] |
| 生物功能 | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. [UniProt] |
| 预测分子量 | 92 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57042 anti-GRP94 antibody [94B9] ICC/IF image
Immunoflorescense: U87MG cell line stained with ARG57042 anti-GRP94 antibody [94B9] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57042 anti-GRP94 antibody [94B9] FACS image
Flow Cytometry: U87MG cell line stained with ARG57042 anti-GRP94 antibody [94B9] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57042 anti-GRP94 antibody [94B9] WB image
Western blot: 1) 25 ng of Recombinant Human GRP94, 40 µg of 2) HeLa cell lysate, 3) Jurkat cell lysate, 4) HepG2 cell lysate stained with ARG57042 anti-GRP94 antibody [94B9] at 1:3000.
