ARG58855

anti-GRK5 antibody

anti-GRK5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes GRK5
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GRK5
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 371-590 of Human GRK5 (NP_005299.1).
偶联标记 Un-conjugated
別名 G protein-coupled receptor kinase 5; EC 2.7.11.16; G protein-coupled receptor kinase GRK5; GPRK5

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:200 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 U-251
实际分子量 68 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14773 Mouse GRK5

GeneID: 2869 Human GRK5

GeneID: 59075 Rat GRK5

基因名称 GRK5
全名 G protein-coupled receptor kinase 5
背景介绍 This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]
生物功能 Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro). [UniProt]
细胞定位 Cytoplasm, Nucleus, Cell membrane, Peripheral membrane protein. [UniProt]
预测分子量 68 kDa
翻译后修饰 Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG58855 anti-GRK5 antibody WB image

    Western blot: 25 µg of U-251 cell lysate stained with ARG58855 anti-GRK5 antibody at 1:1000 dilution.