ARG59543

anti-GRK2 antibody

anti-GRK2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes GRK2
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GRK2
抗原物种 Human
抗原 Synthetic peptide corresponding to a sequence of Human GRK2. (DSDPELVQWKKELRDAYREAQQLVQRVPKMKNK)
偶联标记 Un-conjugated
別名 BETA-ARK1; Beta-adrenergic receptor kinase 1; GRK2; BARK1; EC 2.7.11.15; Beta-ARK-1; G-protein coupled receptor kinase 2

应用说明

应用建议
应用 推荐稀释比
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB0.1 - 0.5 µg/ml
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 110355 Mouse ADRBK1

GeneID: 156 Human ADRBK1

GeneID: 25238 Rat ADRBK1

基因名称 ADRBK1
全名 adrenergic, beta, receptor kinase 1
背景介绍 The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
生物功能 Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. [UniProt]
细胞定位 Cytoplasm. Cell membrane. [UniProt]
预测分子量 80 kDa

检测图片 (6) Click the Picture to Zoom In

  • ARG59543 anti-GRK2 antibody ICC/IF image

    Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG59543 anti-GRK2 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG59543 anti-GRK2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59543 anti-GRK2 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG59543 anti-GRK2 antibody WB image

    Western blot: 50 µg of samples under reducing conditions. Rat spleen, Rat stomach, Mouse lung, Mouse liver and Mouse pancreas lysates stained with ARG59543 anti-GRK2 antibody at 0.5 µg/ml, overnight at 4°C.

  • ARG59543 anti-GRK2 antibody FACS image

    Flow Cytometry: U87 cells were blocked with 10% normal goat serum and then stained with ARG59543 anti-GRK2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG59543 anti-GRK2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59543 anti-GRK2 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG59543 anti-GRK2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59543 anti-GRK2 antibody at 1 µg/ml dilution, overnight at 4°C.