ARG64711
anti-GRIA4 antibody
anti-GRIA4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GRIA4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GRIA4 |
| 抗原物种 | Human |
| 抗原 | C-KKLDQREYPGSETP |
| 偶联标记 | Un-conjugated |
| 別名 | GluA4; GLUR4C; GLUR4; Glutamate receptor ionotropic, AMPA 4; GluR-4; AMPA-selective glutamate receptor 4; GluR4; Glutamate receptor 4; GLURD; GluR-D |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008] |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 101 kDa |
| 翻译后修饰 | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). Phosphorylated at Ser-862 by PRKCG; phosphorylation increases plasma membrane-associated GRI4 expression. |
检测图片 (2) Click the Picture to Zoom In
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ARG64711 anti-GRIA4 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64711 anti-GRIA4 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64711 anti-GRIA4 antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG64711 anti-GRIA4 antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
